A nurse is teaching a newly licensed nurse about collecting a specimen for the universal newborn screening. Which of the following statements should the nurse include in the teaching?

1) obtain informed consent before getting the specimen
2) collect at least 1 mL of urine for the test
3) ensure that the newborn has been receiving feeding for 24 hours prior to obtaining the specimen
4) premature newborns may have a false-negative test due to immature development of liver enzymes